A survey of enzymes which generate or use acetoacetyl thioesters in rat liver.

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Preparation and properties of a liver esterase which hydrolyzes S-acetoacetyldithioloctanoic acid and related thioesters.

The occurrence of thioesterases hydrolyzing thioesters of monothiol coenzymes, e.g. glutathione (l-3) and wenzyme A (P6), is well documented. In the course of experiments on the metabolism of acetoacetyl thioesters (7), it was found that liver enzyme fractions catalyzed the hydrolysis of (f)-S-acetoacetyl6 ,E&dithioloctanoic(dihydrolipoic) acid (Reaction 1) and ( &S-acetyld, Sdithioloctanoic(di...

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The kinetic mechanism and properties of the cytoplasmic acetoacetyl-coenzyme A thiolase from rat liver.

1. Cytoplasmic acetoacetyl-CoA thiolase was highly purified in good yield from rat liver extracts. 2. Mg(2+) inhibits the rate of acetoacetyl-CoA thiolysis but not the rate of synthesis of acetoacetyl-CoA. Measurement of the velocity of thiolysis at varying Mg(2+) but fixed acetoacetyl-CoA concentrations gave evidence that the keto form of acetoacetyl-CoA is the true substrate. 3. Linear recipr...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1983

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)32482-7